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suspension cell culture


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2 replies to this topic

#1 youyou

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Posted 20 July 2009 - 06:22 AM

Hi everyone

i have a question about the optimum number of cells in suspension that can be cultured in multi well plate i.e in a 24 well plate what is the max. no. of cells that can be cultured for a period of 72 hours???????

thanx for your opinions in advance

yusra

#2 calvin*

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Posted 21 July 2009 - 03:31 AM

You can try different cell numbers such as 1X10^4, 5X10^4 and 1X10^5. If clumping is a matter for you you can reduce the cell number further.
"In my opinion, we don't devote nearly enough scientific research to finding a cure for jerks"-Calvin

#3 pito

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Posted 21 July 2009 - 05:13 AM

It depends on what medium you use, what kind of cells etc...

How you incubate..

Best way is to test it yourself, see what concentration fits your experiment.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.





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