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DNA extraction fron HSV-1 infected Vero cell


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#1 piggle

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Posted 18 July 2009 - 10:30 PM

Hi! everyone
I'm new in DNA extraction from Cell culture. ;)

I want to extract viral DNA from HSV-1 infected Vero cell

Pls, tell me
-whether phenol-chloroform is good and will get high yeild DNA.
-Do i have to detach infected vero cell by using trypsin before extraction?
-How many MOI that good for infection?
-Do i collect both supernate and cells for extraction?

Thank so much
:lol:
piggle

#2 bob1

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Posted 19 July 2009 - 04:29 PM

If you are after pure viral DNA, you will be best to do a virus preparation, and then extract DNA from that. Otherwise, infected cells will have the DNA in them and should be OK to extract with phenol:chloroform straight from the dish.

#3 piggle

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Posted 19 July 2009 - 05:20 PM

thank so much :D




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