Hi,
I have 1 lot of Matrigel which I suspect is contaminated. I need to sterilise it somehow, and was thinking of using a 0.22micron filter. Does anyone know if filtered-Matrigel can still be used for human embryonic stem cell culture? I would really rather salvage this Matrigel rather than discard it.
Any tips would be very appreciated. Thanks in advance!
Cheers,
Sylvia
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Can contaminated Matrigel be sterilised using a 0.22micron filter?
Started by Penguiny, Jul 18 2009 07:29 PM
3 replies to this topic
#2
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Veteran
Posted 19 July 2009 - 02:59 AM
Come on Sylvia - if it's contaminated, toss it.
#3
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Enthusiast
Posted 19 July 2009 - 08:47 AM
hi,
having worked with Matrigel myself I can not imagine at all that you even get it through a filter! And i guess you have no idea, if all the proteins pass through or if the composition changed,...I know it is expensive but throw it away!
Stardust
having worked with Matrigel myself I can not imagine at all that you even get it through a filter! And i guess you have no idea, if all the proteins pass through or if the composition changed,...I know it is expensive but throw it away!
Stardust
#4
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Control Panel
Posted 19 July 2009 - 02:17 PM
Sylvia Peng, on Jul 18 2009, 08:29 PM, said:
Hi,
I have 1 lot of Matrigel which I suspect is contaminated. I need to sterilise it somehow, and was thinking of using a 0.22micron filter. Does anyone know if filtered-Matrigel can still be used for human embryonic stem cell culture? I would really rather salvage this Matrigel rather than discard it.
Any tips would be very appreciated. Thanks in advance!
Cheers,
Sylvia
I have 1 lot of Matrigel which I suspect is contaminated. I need to sterilise it somehow, and was thinking of using a 0.22micron filter. Does anyone know if filtered-Matrigel can still be used for human embryonic stem cell culture? I would really rather salvage this Matrigel rather than discard it.
Any tips would be very appreciated. Thanks in advance!
Cheers,
Sylvia
I would buy new matrigel. But even theoratically, I don't think you can filter it through .22 micron pores.
