3 replies to this topic

[Penguiny]

Hi,

I have 1 lot of Matrigel which I suspect is contaminated. I need to sterilise it somehow, and was thinking of using a 0.22micron filter. Does anyone know if filtered-Matrigel can still be used for human embryonic stem cell culture? I would really rather salvage this Matrigel rather than discard it.

Any tips would be very appreciated. Thanks in advance!

Cheers,
Sylvia

[GeorgeWolff]

Come on Sylvia - if it's contaminated, toss it.

[stardust]

hi,

having worked with Matrigel myself I can not imagine at all that you even get it through a filter! And i guess you have no idea, if all the proteins pass through or if the composition changed,...I know it is expensive but throw it away!

Stardust

[cellcounter]

Sylvia Peng, on Jul 18 2009, 08:29 PM, said:

Hi,

I have 1 lot of Matrigel which I suspect is contaminated. I need to sterilise it somehow, and was thinking of using a 0.22micron filter. Does anyone know if filtered-Matrigel can still be used for human embryonic stem cell culture? I would really rather salvage this Matrigel rather than discard it.

Any tips would be very appreciated. Thanks in advance!

Cheers,
Sylvia

I would buy new matrigel. But even theoratically, I don't think you can filter it through .22 micron pores.