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Can contaminated Matrigel be sterilised using a 0.22micron filter?


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3 replies to this topic

#1 Penguiny

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Posted 18 July 2009 - 07:29 PM

Hi,

I have 1 lot of Matrigel which I suspect is contaminated. I need to sterilise it somehow, and was thinking of using a 0.22micron filter. Does anyone know if filtered-Matrigel can still be used for human embryonic stem cell culture? I would really rather salvage this Matrigel rather than discard it.

Any tips would be very appreciated. Thanks in advance!

Cheers,
Sylvia

#2 GeorgeWolff

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Posted 19 July 2009 - 02:59 AM

Come on Sylvia - if it's contaminated, toss it.

#3 stardust

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Posted 19 July 2009 - 08:47 AM

hi,

having worked with Matrigel myself I can not imagine at all that you even get it through a filter! And i guess you have no idea, if all the proteins pass through or if the composition changed,...I know it is expensive but throw it away!

Stardust

#4 cellcounter

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Posted 19 July 2009 - 02:17 PM

Hi,

I have 1 lot of Matrigel which I suspect is contaminated. I need to sterilise it somehow, and was thinking of using a 0.22micron filter. Does anyone know if filtered-Matrigel can still be used for human embryonic stem cell culture? I would really rather salvage this Matrigel rather than discard it.

Any tips would be very appreciated. Thanks in advance!

Cheers,
Sylvia

I would buy new matrigel. But even theoratically, I don't think you can filter it through .22 micron pores.




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