5 replies to this topic

[proteome]

Hi  all,

I am having serious problems in culturing COS-7 cells.. they tend to maintain the same state , as in, they neither grow or die off after getting adhered..morphology is fine..n the intriguin part is that they they are growin in parallel lines? HELP PLEASE  

[Dr Teeth]

proteome, on Jul 17 2009, 09:16 AM, said:

Hi  all,

I am having serious problems in culturing COS-7 cells.. they tend to maintain the same state , as in, they neither grow or die off after getting adhered..morphology is fine..n the intriguin part is that they they are growin in parallel lines? HELP PLEASE  

I find that my COS-7 cells grow quite rapidly and do so in a normal pattern with the expected morphology. I have never seen them fail to grow or grow in "lines."   Two questions, then:
1) Are you using the proper medium?  I use low glucose DMEM supplemented with 10% fetal bovine serum
2) Are you sure these are COS-7 cells?

[stardust]

Hi,

Does your medium contain glutamine? Or do you have to add it? Did you? This was the reason why some of my cells did not grow, thought medium contains l-glutamin /stabilized glutamin but it did not...

Stardust

[Dr Teeth]

stardust, on Jul 18 2009, 07:47 AM, said:

Hi,

Does your medium contain glutamine? Or do you have to add it? Did you? This was the reason why some of my cells did not grow, thought medium contains l-glutamin /stabilized glutamin but it did not...

Stardust

Yes, the medium contains L-glutamine and 110 mg/L sodium pyruvate.

[Dominic]

Dr Teeth, on Jul 20 2009, 03:08 PM, said:

Yes, the medium contains L-glutamine and 110 mg/L sodium pyruvate.

yours might - but its a little too easy to grab the wrong dmem bottle if your new to the game - proteome - you should check

love the idea of growth in parralel lines - like synchronised swimming for cells - you could sell tickets

  dom

[bob1]

Hmmm, parallel line usually means that your cell cultures are being exposed to vibrations - check your incubator for a small vibration and get it sorted out. See attachment.

Attached Files

•  cc_guide_identifing_correcting_common_cell_growth_problem_5_2_03_cls_cc_013w.pdf   493.25K   139 downloads