Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

culturing COS-7 cells


  • Please log in to reply
5 replies to this topic

#1 proteome

proteome

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 17 July 2009 - 05:16 AM

Hi all,

I am having serious problems in culturing COS-7 cells.. they tend to maintain the same state , as in, they neither grow or die off after getting adhered..morphology is fine..n the intriguin part is that they they are growin in parallel lines? HELP PLEASE :)

#2 Dr Teeth

Dr Teeth

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 221 posts
1
Neutral

Posted 17 July 2009 - 06:34 AM

Hi all,

I am having serious problems in culturing COS-7 cells.. they tend to maintain the same state , as in, they neither grow or die off after getting adhered..morphology is fine..n the intriguin part is that they they are growin in parallel lines? HELP PLEASE :)


I find that my COS-7 cells grow quite rapidly and do so in a normal pattern with the expected morphology. I have never seen them fail to grow or grow in "lines." Two questions, then:
1) Are you using the proper medium? I use low glucose DMEM supplemented with 10% fetal bovine serum
2) Are you sure these are COS-7 cells?

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#3 stardust

stardust

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 60 posts
1
Neutral

Posted 18 July 2009 - 03:47 AM

Hi,

Does your medium contain glutamine? Or do you have to add it? Did you? This was the reason why some of my cells did not grow, thought medium contains l-glutamin /stabilized glutamin but it did not...

Stardust

#4 Dr Teeth

Dr Teeth

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 221 posts
1
Neutral

Posted 20 July 2009 - 06:08 AM

Hi,

Does your medium contain glutamine? Or do you have to add it? Did you? This was the reason why some of my cells did not grow, thought medium contains l-glutamin /stabilized glutamin but it did not...

Stardust


Yes, the medium contains L-glutamine and 110 mg/L sodium pyruvate.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley

#5 Dominic

Dominic

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 47 posts
2
Neutral

Posted 20 July 2009 - 06:19 AM

Yes, the medium contains L-glutamine and 110 mg/L sodium pyruvate.


yours might - but its a little too easy to grab the wrong dmem bottle if your new to the game - proteome - you should check

love the idea of growth in parralel lines - like synchronised swimming for cells - you could sell tickets

:lol: dom

#6 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,829 posts
414
Excellent

Posted 21 July 2009 - 07:29 PM

Hmmm, parallel line usually means that your cell cultures are being exposed to vibrations - check your incubator for a small vibration and get it sorted out. See attachment.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.