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Ponceau Staining Question


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#1 cwong1215

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Posted 16 July 2009 - 11:54 AM

Hi Guys,

Quick ponceau question. I was running behind yesterday when i started my western and didn't have a chance to ponceau stain it before i did my blocking step overnight. Is there any chance I can still stain it after I strip the membrane of my first set of primary/secondary antibodies and get an idea about the protein per lane or will the blocking obscure that?

Thanks.

#2 bob1

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Posted 16 July 2009 - 05:11 PM

blocking will obscure. Ponceau binds to protein and you use protein for blocking...




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