1 reply to this topic

[cwong1215]

Hi Guys,

Quick ponceau question.  I was running behind yesterday when i started my western and didn't have a chance to ponceau stain it before i did my blocking step overnight.  Is there any chance I can still stain it after I strip the membrane of my first set of primary/secondary antibodies and get an idea about the protein per lane or will the blocking obscure that?

Thanks.

[bob1]

blocking will obscure. Ponceau binds to protein and you use protein for blocking...