3 replies to this topic

[yja97]

Hi. I tried to maesure protein conc. by Bradford assay.
I got satisfactory standard curve, but I found something wierd.
My sample with bradford reagent is too bright. Actually I found the mixture had highest absorbance at 625nm.

What's wrong with my sample? Actually I avoided using detergents.
Thanks in advance.

[fishdoc]

yja97, on Jul 16 2009, 10:20 AM, said:

Hi. I tried to maesure protein conc. by Bradford assay.
I got satisfactory standard curve, but I found something wierd.
My sample with bradford reagent is too bright. Actually I found the mixture had highest absorbance at 625nm.

What's wrong with my sample? Actually I avoided using detergents.
Thanks in advance.

Could it be too concentrated? Try diluting it 1 in 10 or more.

[yja97]

Thanks for reply, fishdoc.
But I don't think my protein has high conc. I'm expecting around 10 ug/ml.

[genehunter]

What else are in the buffer?  Detergent components, such as Triton X 100 can interfere the results.