Edited by QQ_lin, 16 July 2009 - 05:31 AM.
Culture of soil bacteria
Posted 16 July 2009 - 05:28 AM
Posted 16 July 2009 - 05:48 AM
I am now involving a study regarding soil bacteria. Is it compulsory for me to do serial dilutions for the soil sample before pouring the aliquot onto the agar medium? And what is the difference between mixing the soil sample with Phosphate Buffer Saline (PBS) instead of using sterile distilled water? Thanks.
You do not really need to make a dilution, it all depends on how many cells you have in your sample.
You could make a dilution and plate out every dilution and see till what dilution you still have a good growth on your plates.
I you have almost no cells in your sample, then you do not want to dilute.
And PBS could favor your cells(its isotonic), but all depends on what cells you have.
You could try one sample with PBS and another one with distilled water. If both works out the same then simply use the cheapest one.
Edited by pito, 16 July 2009 - 06:08 AM.
If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.
Posted 16 July 2009 - 08:54 AM
Gratuitous comparison to Lucozade aside, what do you want to understand about the microbes in the soil sample? Total culturable count? Many of the bacterial species in sopil won't grow well or at all in culture and fungal biomass is not correctly estimated by culture counts.
Edited by GeorgeWolff, 16 July 2009 - 08:59 AM.
Posted 17 July 2009 - 04:22 AM
Posted 17 July 2009 - 11:54 AM
didnt lucozade sell itself as being isotonic? or is that the wrong drink?
I think it depends on what "kind" you buy because Lucozade Hydro Active is not isotonic but hypotonic.
Why do you come up with this Lucozade anyway?
Is it cheaper to buy then to make some PBS?
Posted 18 July 2009 - 03:03 AM
Ingredients in Lucozade
Carbonated Water, Glucose Syrup (26%), Citric Acid, Lactic Acid, Flavourings (including Caffeine), Preservatives (Sodium Benzoate, Sodium Bisulphate), Antioxidant (Ascorbic Acid), Colour (Sunset Yellow).
But back to the initial question - soil itself will offer more solutes than the little in PBS and what would be the purpose of buffering in dilution?
Stil - as pito said - try it both ways.
Posted 18 July 2009 - 07:13 AM
Posted 18 July 2009 - 07:19 AM
Really thanks for your reply, Pito and also the others. I really appreciate it. Actually I want to see what types of bacteria colonies that I can get from the soil sample that I collected from different areas so that I can do isolation for those that I am interested in.
If that is the case, then do not dilute.
See what you get first and then you can start thinking about making dilutions etc..
Posted 18 July 2009 - 11:33 AM
Posted 27 July 2009 - 01:56 AM
Roald Sorheim, Vigdis Lid Torsvik, and Jostein Goksoyr.
Posted 27 July 2009 - 07:06 AM