5% vs 10% CO2 (with their corresponding media)
Posted 16 July 2009 - 03:48 AM
We are starting in our cell culture lab, and from time to time basic questions arise. Right now we are preparing a SH-SY5Y culture, but the question is a generic one.
We have realized that depending on the paper, researchers use one or another media for the same cell lines and similar experiments. Usually in the Material and Methods section the %CO2 is not mentioned. Each media has its own bicarbonate concentration and thus its own optimal % CO2 (e.g. EMEM for 5% and DMEM for 10%), but from some suppliers we may have DMDM with lower amount of bicarbonate and vice versa, so no % may be a priori excluded.
My question is: If the bicarbonate concentration and the %CO2 of the incubator are harmonized, the pH should be the desired 7.4, right? In this case, is the CO2 atmospheric content a relevant factor for the cell behaviour?? As far as I understand, in a pH 7.4 media the content of CO2/bicarbonate should be the same, regardless the atmospheric CO2 concentration...
Did I explain my question clearly enough?
I will appreciate any explanation, either theorical or practical ones.
Posted 16 July 2009 - 06:11 AM
then heaven will be yours, before you meet your end
Posted 16 July 2009 - 06:45 AM
My question is a bit broader, based on these different protocols for similar experiments. In your example of myeloma line, did you change the recommended media according to the %CO2 or did you use the same media all the time? If you did the second one, then the pH was different and the responsible for the different grow pattern; if not, it was the total %CO2 the responsible one, and that would be the answer to my general question: if CO2 per se plays a role.
Posted 16 July 2009 - 04:36 PM
It is entirely possible to grow cells at the "wrong" %CO2, the pH isn't too different, usually a difference of about 0.2-0.4 pH units between 5 and 10%
Posted 27 December 2012 - 01:41 AM