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2D electrophoresis


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#1 T C

T C

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Posted 16 July 2009 - 01:00 AM

Hello,

I was reading a protocol to prepare cell lysates to be analyzed by 2D-electrophoresis. The protocol describes spiking of the lysate with beta-lactoglobulin A. Is this step necessary? and how does it help?

Best,
Tarun

#2 T C

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Posted 16 July 2009 - 01:11 AM

My mistake...they were spiking it with beta-lactoglobulin to detect and see if their method actually differentiates between control and spiked samples (it was an exp)

How do I delete a topic?

Best,
TC

Hello,

I was reading a protocol to prepare cell lysates to be analyzed by 2D-electrophoresis. The protocol describes spiking of the lysate with beta-lactoglobulin A. Is this step necessary? and how does it help?

Best,
Tarun






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