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contamination


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2 replies to this topic

#1 thinsu

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Posted 15 July 2009 - 11:27 PM

Hi all,
i have a problem that there is signal in negative control in real time PCR?why is it caused?i use new reagents but contamination is found.what should i do?please help me.
best regards,
thinsu

#2 ivanbio

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Posted 16 July 2009 - 07:23 AM

On possible cause is the formation of primer dimers. For this, one of the best (and most time efficient) approaches is to redesign your assay and try again. I typically design at least three assays, test them all together, and chose the best one.

Good luck

Ivan
Carlsbad, CA

#3 thinsu

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Posted 21 July 2009 - 07:01 AM

On possible cause is the formation of primer dimers. For this, one of the best (and most time efficient) approaches is to redesign your assay and try again. I typically design at least three assays, test them all together, and chose the best one.

Good luck

thanks a lot. i 'll try it.
best regards,
thinsu




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