Posted 15 July 2009 - 11:27 PM
i have a problem that there is signal in negative control in real time PCR?why is it caused?i use new reagents but contamination is found.what should i do?please help me.
Posted 16 July 2009 - 07:23 AM
Posted 21 July 2009 - 07:01 AM
thanks a lot. i 'll try it.
On possible cause is the formation of primer dimers. For this, one of the best (and most time efficient) approaches is to redesign your assay and try again. I typically design at least three assays, test them all together, and chose the best one.