I am new to this field. I've read the 2004 cell paper on H3K4me1/2 demethylase LSD1 identification, and have a question on one of the experiment.
In this experiment, (figure1C, D), the author transfected G4-LSD1 and G4DBD plasmids into HeLa cells together with G4TK-Luc reporter gene. The result shows that reporter luciferase activity decreased after G4LSD1 transfection. I guess that the promoter of this reporter gene must locate in the nucleus and be bound with K4 methylated histone H3, so that LSD1 demethylase activity can act to repress the reporter gene.
I am confused about this point. How can the transfected G4TK-Luc reporter gene get into the nucleus and associate with K4 methylated histone? Or there is anthor explanation about this experiment?
Edited by bsksln, 16 July 2009 - 01:26 AM.