I had a quick question regarding agarose gel electrophoresis.
So I have my gel made, and I have my DNA/dye mixture (I usually use a ratio of 1uL 5x loading dye: 4uL DNA), and then I load the mixture into the wells. So far so good.
I start running the gel. So far so good, then maybe five minutes in, the dye starts leaking out of the well/gel. At least some of the mixture is still traveling as usual through the gel through, because in the end, I still get visible bands, but it makes me wonder just how much DNA is randomly leaking out.
Sometimes the DNA mixture doesn't leak out of the well, but rather it is running just fine, halfway through the gel, and then the mixture leaks out through the top of the gel.
My question is basically, "What am I doing wrong? Is the loading dye concentration too low? Is the gel of uneven thickness?" (Okay that's like three questions)
I was just wondering if anyone had any similar issues, and if he or she successfully fixed the problem.
Thanks for reading!
Edited by xCj425x, 15 July 2009 - 10:36 AM.