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cloning problem


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#1 xyz

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Posted 15 July 2009 - 09:05 AM

Hi everyone,
I am having problem in cloning of PCR product (1.4 kb) into vector (PUC19-2.6 kb). I am using BamH1 restriction sites for cloning. After ligation and transformation I am not getting any colony in both ligation product and negative control as well. I tried various combination of insert: vector, but futile. i didn't get even a single colony. Any suggestions? I am working on this from a month------------------

#2 stardust

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Posted 15 July 2009 - 09:16 AM

Hi, could you give more details of what you have done? Purified plasmid from gel? Digestion protocol? Ligation protocol?...otherwise we can't help you...

Stardust




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