I am having problem in cloning of PCR product (1.4 kb) into vector (PUC19-2.6 kb). I am using BamH1 restriction sites for cloning. After ligation and transformation I am not getting any colony in both ligation product and negative control as well. I tried various combination of insert: vector, but futile. i didn't get even a single colony. Any suggestions? I am working on this from a month------------------
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