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His-tag antibody for western


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#1 kikieb

kikieb

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Posted 15 July 2009 - 07:50 AM

I created a vector to express a secreted his-tagged protein. Can anyone recommend an antibody for western blotting to confirm expression?

I read about some antibodies only detecting his-tags at the N-terminus or C-terminus or with a particular amino acid after the tag. Some antibodies online say they are good for proteins expressed from specific vectors (pET etc), but our vector is homemade and I included 6X-His in my insert to tag the protein.

Any help is greatly appreciated!!
Kiki




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