Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DNA isolation from tissue !!SOS!!


  • Please log in to reply
1 reply to this topic

#1 olga_m

olga_m

    member

  • Active Members
  • Pip
  • 20 posts
0
Neutral

Posted 15 July 2009 - 02:15 AM

I used to isolate DNA from cells using a phenol/chloroform method.
Now I want to to do the same with tissue samples. My tissue is frozen and powdered.
But I have no idea about the ammount of tissue I should use. :)
Can anyone help me with this?
I would appreciate it very much!
Thank u! :D

#2 soldierhxc

soldierhxc

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 15 July 2009 - 07:09 AM

That depends a lot on the method u are using, and the amount and quality of the DNA you need for next experiments, IŽd try 1gr and 0.5gr. That should work.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.