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Human Serum help


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3 replies to this topic

#1 laska

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Posted 14 July 2009 - 10:56 PM

Hey
I need to culture my cells in 10% human serum. However every time I mix it with medium it precipitates. I tried all techniques but nothing. What to do now. The medium when transfer to +4C looks like gelly :)

MJL

#2 little mouse

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Posted 15 July 2009 - 01:05 AM

this sounds really strange. Where did you get your serum from? I never had such problem with human serum (from companies or home made)

#3 klinmed

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Posted 15 July 2009 - 07:43 AM

Hey
I need to culture my cells in 10% human serum. However every time I mix it with medium it precipitates. I tried all techniques but nothing. What to do now. The medium when transfer to +4C looks like gelly :D

MJL

Just a thought:

You are using serum and not plasma?
If you are making your own serum, remember to collect blood without anticoagulant, allow to clot for at least 30 minute at room temp. before harvesting serum.

Hope this helps

#4 ChrisHarris

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Posted 07 August 2009 - 03:04 AM

I agree with the above post.

Medium + plasma = clot (I grow in plasma clots all the time)

Cheers
Chris




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