I am confused by something....
If a cell lysis buffer is supposed to give you whole cell lysate (membrane and cytosolic proteins) why is there cell debris? What is it?
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bob1
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Posted 14 July 2009 - 04:11 PM
bits that resisted going into solution, especially insoluble proteins and bits bound up in DNA.
#3
miBunny
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Posted 14 July 2009 - 06:13 PM
the cell debris is the parts of the cell that are not soluble in the detergent. A lot of the cytoskeletal components and membrane components will not solubilize in the detergent solution and thus are pelleted out.
