Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

General cell lysis question


  • Please log in to reply
2 replies to this topic

#1 Trying-2-Graduate

Trying-2-Graduate

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 14 July 2009 - 12:44 PM

I am confused by something....

If a cell lysis buffer is supposed to give you whole cell lysate (membrane and cytosolic proteins) why is there cell debris? What is it?

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,628 posts
389
Excellent

Posted 14 July 2009 - 04:11 PM

bits that resisted going into solution, especially insoluble proteins and bits bound up in DNA.

#3 miBunny

miBunny

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 130 posts
1
Neutral

Posted 14 July 2009 - 06:13 PM

the cell debris is the parts of the cell that are not soluble in the detergent. A lot of the cytoskeletal components and membrane components will not solubilize in the detergent solution and thus are pelleted out.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.