I am dialysising using PBS at 4degrees.
I haven't done dialysis before so maybe I'm making a rookie mistake, but it seems very strange to me.
I can do dialysis with just about any buffer. The salt concentration of my sample isn't important, so if anyone has any suggestions I'd be very greatful.
I should also say that I'm not trying to denature the sample at all. I'd like to try and keep the proteins fully natured.
Edited by jaknight, 14 July 2009 - 12:16 PM.