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GC rich DNA sequencing


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#1 subtelo

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Posted 14 July 2009 - 10:17 AM

Does anyone have any successful experience regarding sequencing GC rich (~70%) DNA sequence. I tried with 3% DMSO. Sequencing always stop once meet GC rich region. Thanks a lot.

#2 mdfenko

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Posted 14 July 2009 - 11:33 AM

try 1M betaine
talent does what it can
genius does what it must
i do what i get paid to do

#3 subtelo

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Posted 14 July 2009 - 12:15 PM

try 1M betaine

I also see this online too. Do you change anything of sequencing cycle? Thanks.

#4 mdfenko

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Posted 15 July 2009 - 07:38 AM

nope. add the betaine and run as usual.

i often tell investigators to increase the number of cycles to maximum when dealing with weak reactions.
talent does what it can
genius does what it must
i do what i get paid to do




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