Does anyone have any successful experience regarding sequencing GC rich (~70%) DNA sequence. I tried with 3% DMSO. Sequencing always stop once meet GC rich region. Thanks a lot.
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#2
mdfenko
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Posted 14 July 2009 - 11:33 AM
try 1M betaine
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
subtelo
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Posted 14 July 2009 - 12:15 PM
mdfenko, on Jul 14 2009, 03:33 PM, said:
try 1M betaine
#4
mdfenko
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Posted 15 July 2009 - 07:38 AM
nope. add the betaine and run as usual.
i often tell investigators to increase the number of cycles to maximum when dealing with weak reactions.
i often tell investigators to increase the number of cycles to maximum when dealing with weak reactions.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
