Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Rnase for cell cycle


  • Please log in to reply
5 replies to this topic

#1 canotto

canotto

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 45 posts
1
Neutral

Posted 14 July 2009 - 08:37 AM

Do you know for how long Rnase 100 micrograms/ml can be stored at +4?

#2 illuminated

illuminated

    member

  • Active Members
  • Pip
  • 19 posts
0
Neutral

Posted 23 July 2009 - 06:37 AM

I don't have any idea but why don't you freeze it? At -20 it can be stored for ages!

#3 canotto

canotto

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 45 posts
1
Neutral

Posted 24 July 2009 - 04:47 AM

I don't have any idea but why don't you freeze it? At -20 it can be stored for ages!


thanks, I have some aliquotes that are already at -20, but the concentration is much higher . Unfortunately I made too big aliquotes, so when I dilute 1 aliquote to the working concentration, that is 100 microg/ml, I don't know if I can just keep in the fridge ( and for how many days?) or refreeze. what do you do usually?

#4 illuminated

illuminated

    member

  • Active Members
  • Pip
  • 19 posts
0
Neutral

Posted 27 July 2009 - 02:25 AM

I don't have any idea but why don't you freeze it? At -20 it can be stored for ages!


thanks, I have some aliquotes that are already at -20, but the concentration is much higher . Unfortunately I made too big aliquotes, so when I dilute 1 aliquote to the working concentration, that is 100 microg/ml, I don't know if I can just keep in the fridge ( and for how many days?) or refreeze. what do you do usually?


I made 10 ml of the solution of the right concentration, froze them in a 15 ml tube and defrost it every time I need it to add 50 ul to my samples. Than I freeze it again. So I don't aliquot it at all and it works fine.

#5 canotto

canotto

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 45 posts
1
Neutral

Posted 27 July 2009 - 02:29 PM

I don't have any idea but why don't you freeze it? At -20 it can be stored for ages!


thanks, I have some aliquotes that are already at -20, but the concentration is much higher . Unfortunately I made too big aliquotes, so when I dilute 1 aliquote to the working concentration, that is 100 microg/ml, I don't know if I can just keep in the fridge ( and for how many days?) or refreeze. what do you do usually?


I made 10 ml of the solution of the right concentration, froze them in a 15 ml tube and defrost it every time I need it to add 50 ul to my samples. Than I freeze it again. So I don't aliquot it at all and it works fine.


thanks. I think I will waste this ( that is >1 month that is in the fridge) and make another 100 ug/ml solution and do like you do

#6 Brachya

Brachya

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 04 March 2010 - 08:28 AM

My lab mate used to keep Rnase at RT for ages w/o any problems
so I don't think you should worry to much




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.