I had prepared several SDS-PAGE gels without difficulty but recently I have encountered a problem. The gel polymerizes well but when I detach it from clips and pull out the lower spacer, bubbles form between the gel and glass plates and then they come up and become trapped between the stacking and resolving gels; very distorted appearance! I changed every ingredient of the gel but it didn't make any difference. Does anyone know why it happens and what I can do to avoid it?
Edited by mtrnbh, 14 July 2009 - 06:00 AM.