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transformation of fd tet vector


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#1 anonymous

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Posted 14 July 2001 - 09:00 PM

i am having problems getting good efficiency of transformation of fd tet TA 74 phage display vector (9.5 kbp), using DH5alpha host cells. Although it is a phage display vector and can produce plaques if we use F' cells as host, we are currently interested in getting colonies (by transformation). Any tips/ solutions to this problem will be appreciated.




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