Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Protein glycosylation


  • Please log in to reply
2 replies to this topic

#1 ram

ram

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 96 posts
0
Neutral

Posted 14 July 2009 - 02:57 AM

I have isolated complete open reading frame of a gene from plant and now I want to express it recombinantly. For the proteins which undergo post-translational glycosylation, euokaryotic expression systems are suggested. In such case how do I know whether the protein that I am going to express in vitro is glycosylated in planta or not? There is no such information available about my protein of interest in the literature. If I isolate this protein from plant, is there any way know where it is glycosylated or not?
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#2 HomeBrew

HomeBrew

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 930 posts
16
Good

Posted 14 July 2009 - 04:56 AM

Yes -- you can use a general glycoprotein staining kit, like the Pro-Q Emerald 300 Glycoprotein Stain Kit from Invitrogen (see here).

If you isolate the protein from the plant, you can see if it's glycosylated in planta with this kit. Whether it will be glycosylated in another background is a question.

#3 Prep!

Prep!

    Am I me???!!!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 517 posts
4
Neutral

Posted 26 October 2009 - 08:52 PM

Yes -- you can use a general glycoprotein staining kit, like the Pro-Q Emerald 300 Glycoprotein Stain Kit from Invitrogen (see here).

If you isolate the protein from the plant, you can see if it's glycosylated in planta with this kit. Whether it will be glycosylated in another background is a question.


also thermo has a glycoprotein staining kit!!!
Support bacteria - They are the only culture some people have!!!
Cheers!!!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.