only monomer immunoprecipitates
Posted 13 July 2009 - 01:47 PM
I am using Protein A/G Plus Agarose, and the antibody is rabbit polyclonal. I incubate the antibody in the column for 1 hr, incubate crosslinker for 1 hr, and incubate the homogenate in the column overnight. These are done at 4C while rocking.
I am not sure if I should optimize this protocol or if the problem is actually caused by something else.
Posted 13 July 2009 - 05:26 PM
Good luck! These things can be a pain to work out!
Posted 14 July 2009 - 11:16 AM
Your ideas are brilliant and as I think every time I attempt science, I wish the answer could just slap me in the face..