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DAPI staining


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#1 yacon project

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Posted 13 July 2009 - 12:23 AM

Hello, everybody :P
I have just begin my experiment about apoptosis assay using DAPI staining. But, I have some problems about How to classify the character of normal cell and apoptotic cell. I confused about the character of nuclei condensation. So, I attached the three images of treatment groups
en0.jpg uve0.jpg unknow2_0.jpg .
Last picture is a control group. Controlb16_trail2.jpg
Thank you to you all in advance
Best regards

Edited by yacon project, 13 July 2009 - 12:37 AM.


#2 Dominic

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Posted 13 July 2009 - 05:51 AM

stain with anti active caspase III + dapi and you'll see the difference between the cells

#3 cellcounter

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Posted 13 July 2009 - 11:33 AM

Hello, everybody B)
I have just begin my experiment about apoptosis assay using DAPI staining. But, I have some problems about How to classify the character of normal cell and apoptotic cell. I confused about the character of nuclei condensation. So, I attached the three images of treatment groups
en0.jpg uve0.jpg unknow2_0.jpg .
Last picture is a control group. Controlb16_trail2.jpg
Thank you to you all in advance
Best regards

You should see smaller, fragmented nuclei (apoptotic bodies) as compared to control. Best thing is to follow the previous response.

#4 yacon project

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Posted 17 July 2009 - 10:42 PM

stain with anti active caspase III + dapi and you'll see the difference between the cells

Thank you so much for your answer. you mean only dapi it's not suitable for apoptosis assay right. But,why I have read many paper using this method?

#5 Dominic

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Posted 20 July 2009 - 04:45 AM

the only thing i trust a nuclear stain to do is show me where the nucleus is

if you see more than 20 cells they start to blend into one - you need a clear marker - not a vague hint in the nucleus

maybe there are people out there using the nuclear method - real question is why trust their work when its obviously so subjective

dom




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