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Is molecular biology grade DMSO good enough for cell stock?


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#1 zzll

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Posted 11 July 2009 - 05:33 PM

Hi all,

I want to make insect cell stocks. I have a bottle of molecular biology DMSO in the lab. So I'd like to know if tissue culture grade DMSO is required.


Many thanks.

zzll

#2 klinmed

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Posted 11 July 2009 - 11:28 PM

Hi all,

I want to make insect cell stocks. I have a bottle of molecular biology DMSO in the lab. So I'd like to know if tissue culture grade DMSO is required.


Many thanks.

zzll

Molecular biology DMSO will be fine. In fact any reagent grade material will work well.

In my experience what is important is that the DMSO should not be from a partially full, old bottle, that has been hanging around the lab for years.
When DMSO is exposed to air a product (oxidation?) is formed that significantly increases itīs toxicity to cells.

I usually get a small unopened bottle, make 2 - 5 ml aliquots in tightly capped glass tubes (usually use plain blood collection tubes, "vacutainers"), and store at -20 oC.

You can always do a trial with your DMSO. Make a few vials of stock, store in liquid nitrogen for a day or so and thaw. Do a viability after overnight culture. I usually find > 80%.

Hope this helps

#3 zzll

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Posted 12 July 2009 - 04:41 AM

Hi all,

I want to make insect cell stocks. I have a bottle of molecular biology DMSO in the lab. So I'd like to know if tissue culture grade DMSO is required.


Many thanks.

zzll

Molecular biology DMSO will be fine. In fact any reagent grade material will work well.

In my experience what is important is that the DMSO should not be from a partially full, old bottle, that has been hanging around the lab for years.
When DMSO is exposed to air a product (oxidation?) is formed that significantly increases itīs toxicity to cells.

I usually get a small unopened bottle, make 2 - 5 ml aliquots in tightly capped glass tubes (usually use plain blood collection tubes, "vacutainers"), and store at -20 oC.

You can always do a trial with your DMSO. Make a few vials of stock, store in liquid nitrogen for a day or so and thaw. Do a viability after overnight culture. I usually find > 80%.

Hope this helps


This really helps.

Yes, the DMSO at hand is new and never opened. But it's a good idea to make aliquots. I don't have such fancy tubes. The glass tubes in our lab are either w/o caps or culture tubes with plastic caps.

I know polyethylene is insoluble in DMSO. Has anyone tried to store DMSO in such tubes?




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