Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?)
Posted 11 July 2009 - 08:55 AM
Also, if I re-transformed and plated the bacteria today (at the latest, 7 PM), could I leave the plates in the incubator until Monday morning? I don't want to starve my cells again LOL.
I'm only a lowly summer intern who doesn't have access to the autoclave on weekends, so I'd have to wait until Monday to make new agar, and if I incubated on Monday, I'd have to wait until Tuesday to pick colonies and submit stuff for sequencing. And I want to get as much stuff done was possible, because I only have a couple more weeks (ah!!), so I need to submit ASAP.
Thanks for any help!
Posted 12 July 2009 - 06:55 PM
I know you are stressed about losing a day or two but it is better to do things properly from the start rather than going ahead anyway and then finding a problem further down the track and having to start again, losing even more time.
Good luck with your project
Posted 13 July 2009 - 03:42 PM
Could've definitely used it today - ugh, what a horrible Monday.
I forgot that I poured a couple extra plates to begin with, and I just used those, the problem never even existed! Haha good advice though, I'll remember it.
Posted 13 July 2009 - 10:36 PM