It was very difficult to subculture HaCaT
Posted 10 July 2009 - 11:54 PM
but it was very difficult to subculture.
Does anybody have any suggestions as to what is causing this problem of the cells
or has anybody had the same problems and managed to overcome them?
Ex: change trypsin concentrate(%) or add something to medium and PBS buffer.
Thanks for helping!
Posted 13 July 2009 - 06:02 AM
if so - no probs whatsoever
your questions a bit vague - whats the problem - do they shout obcenities at you?
Posted 10 October 2012 - 03:17 PM