2 replies to this topic

[Clare]

Hey everyone

Just wanted to pick your brains about a lil' prob our lab has been having with Trizol.

I have isolated RNA using Trizol for years and years and have never had a problem. A couple of months ago, our nanodrop curves started looking awful after isolation. We suddenly get high absorbance at about 220nm. As I understand it, this is phenol or guanidine contamination...If I precipitate the RNA, then the curves are lovely. But what worries me is that this pesky 220nm peak persists even with a new batch of Trizol, chloroform etc etc.

Does anyone have any ideas why we are suddenly having trouble with Trizol???

Have a great weekend

Clare

[mdfenko]

check the lot#'s of the batches with which you are experiencing this problem.

then, report to the supplier.

[Clare]

I'm not convinced it's a batch problem as I am having the issue with 2 different bottles of Trizol, purchased approx. 6 months apart.
But can try my luck anyway
C

mdfenko, on Jul 10 2009, 04:48 PM, said:

check the lot#'s of the batches with which you are experiencing this problem.

then, report to the supplier.