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Trizol issues!


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2 replies to this topic

#1 Clare

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Posted 10 July 2009 - 07:25 AM

Hey everyone :)

Just wanted to pick your brains about a lil' prob our lab has been having with Trizol.

I have isolated RNA using Trizol for years and years and have never had a problem. A couple of months ago, our nanodrop curves started looking awful after isolation. We suddenly get high absorbance at about 220nm. As I understand it, this is phenol or guanidine contamination...If I precipitate the RNA, then the curves are lovely. But what worries me is that this pesky 220nm peak persists even with a new batch of Trizol, chloroform etc etc.

Does anyone have any ideas why we are suddenly having trouble with Trizol???

Have a great weekend B)

Clare

#2 mdfenko

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Posted 10 July 2009 - 07:48 AM

check the lot#'s of the batches with which you are experiencing this problem.

then, report to the supplier.
talent does what it can
genius does what it must
i do what i get paid to do

#3 Clare

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Posted 10 July 2009 - 07:57 AM

I'm not convinced it's a batch problem as I am having the issue with 2 different bottles of Trizol, purchased approx. 6 months apart.
But can try my luck anyway B)
C

check the lot#'s of the batches with which you are experiencing this problem.

then, report to the supplier.






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