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Subculture of Vero cells


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#1 THE ROCK

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Posted 10 July 2009 - 07:04 AM

Hi friends, can any one suggest for culturing vero cell line. I use Invitrogen IMDM 10% FBS containing 3 gm/ L sod. Bic.I used to split the confluent flask t0 1:1. Daily it become confluent. after 3-4 Passages, these cells has some granular particle, form vacoules and didn't grow thereafter. Why these granular formation occurs. How to avoid it.

#2 bob1

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Posted 12 July 2009 - 04:37 PM

Your cells have senesced, culture at a lower density, and ensure they don't become confluent.




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