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how to put in evidence protein after IP


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#1 gigi

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Posted 09 July 2009 - 05:53 PM

Hello there !!

So after many times, my IP is working is coming down clean :wacko:,

now I have a question for who has more experience than me.

how can I "stain' all the protein come down with the IP?

I tried with the ponceau but I don't think it's strong enough !!

is there any ways to put evidence the protein pulled down using an HRP method?

thank you for your help..

G.

#2 gigi

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Posted 10 July 2009 - 01:45 PM

Nobody has an Idea ?!

#3 cellcounter

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Posted 10 July 2009 - 02:04 PM

Nobody has an Idea ?!

Silver staining and Sypro Ruby staining methods are more sensitive for protein detection.




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