I am doing GST pull down assays. The GST fusion protein is not gets purified singly after incubation with the beads. And when I do the pull down using the in vitro synthesized protein, negative control (GST only) also shows bands. How can I stop the negative (GST only) from interacting with my protein. Any idea or protocol about running a preparatory gel is very welcome.
Hope to hear soon.
Edited by nirajm, 09 July 2009 - 12:49 PM.