Plasmid DNA isolation
Posted 09 July 2009 - 02:16 AM
Any suggestions would be welcomed
Posted 09 July 2009 - 04:44 AM
Posted 09 July 2009 - 04:58 AM
Thanks for the reply. But last time when i used 3M sodium acetate for miniprep, i still got the plasmid DNA which was Ok and even sequencing result is fine. Though i never tried doing downstream proceesing like restriction digestion and transcription.
I feel more discussion on this will be helpful.
Posted 09 July 2009 - 05:37 AM
Posted 09 July 2009 - 06:15 AM
When i made the post, that time i did not have my sequencing results with me , before using the solution for next time i was doubtful about the use of 2 cations and i feel its better to discuss doubts in this forum...and then felt sharing the experience as well.
Posted 09 July 2009 - 08:12 AM