Hi there!
I was wondering whether someone has experience in how to check experimentally (and easily) whether a given protein is able to ubiquitinate histones?
Thank you very much!
cheers!
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Posted 23 July 2009 - 08:04 AM
XXy, on Jul 9 2009, 08:13 AM, said:
Hi there!
I was wondering whether someone has experience in how to check experimentally (and easily) whether a given protein is able to ubiquitinate histones?
Thank you very much!
cheers!
I was wondering whether someone has experience in how to check experimentally (and easily) whether a given protein is able to ubiquitinate histones?
Thank you very much!
cheers!
I don't have any experience in this but can you overexpress your protein in an appropriate cell line and use an anti-ubiquitin antibody to show that there is an increase in histone ubiquitination?
(Not sure if this is a valid idea or not??)
P
