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Histone ubiquitination


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#1 XXy

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Posted 08 July 2009 - 11:13 PM

Hi there!

I was wondering whether someone has experience in how to check experimentally (and easily) whether a given protein is able to ubiquitinate histones?

Thank you very much!

cheers!

#2 Penguin

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Posted 23 July 2009 - 08:04 AM

Hi there!

I was wondering whether someone has experience in how to check experimentally (and easily) whether a given protein is able to ubiquitinate histones?

Thank you very much!

cheers!


I don't have any experience in this but can you overexpress your protein in an appropriate cell line and use an anti-ubiquitin antibody to show that there is an increase in histone ubiquitination?

(Not sure if this is a valid idea or not??)
P




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