ELISA plate layout
Posted 08 July 2009 - 08:07 PM
Posted 09 July 2009 - 01:28 AM
The kit will mostly give also a plate setup and always every in duplo, standards controls and samples.
The dilution you need will also be recommended in the kit, whenever I use a kit for the first time I almost always follows the given instructions without any change.
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".
Posted 09 July 2009 - 08:49 AM
Posted 09 July 2009 - 07:02 PM
Posted 13 July 2009 - 12:24 PM
Posted 17 August 2009 - 05:41 PM
In fact, i wonder i shd use duplicate or triplicate? the reason why i use duplicate is because i want to save wells, especially when some samples are out of ranged and need dilution and re-do. Therefore, i thought i can run all my samples in duplicate first, and when one of my samples, the variation is too wide i still can do another extra well next time (to replace the one with high variation value). Do you think i can make it this way? or does the result actually can be different even using same samples, but analyzed in different time? Thanks
Posted 18 August 2009 - 05:13 AM
I have started to do elisa recently but the most i found useful was to do the elisa standards in triplicated of 12point serial dilution with a start concentration of 1ug/ml , and do the samples in duplicates, and last row use for the blank
This will help you to know the conentration of your standards are of the right concentration which would be easy to analyse the samples.
I hope this make sence
Posted 18 August 2009 - 11:37 AM