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#1 preeti_RNA

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Posted 08 July 2009 - 05:11 AM

HI ALL,

IF anyone can suggest me the protocol for obtaining highly purified DNA sample for transcription and transcription protocol as well
I am using Nucleobond Kit protocol for plasmid DNA preparation, and for transcription, i used linearized fragment post-elution from the agarose gel. If anyone has suggestions. Please write about it


Thanks

Preeti

#2 molgen

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Posted 08 July 2009 - 06:14 AM

The best way to get highly purified DNA samples is by using a Column witch you are doing.
For a protocol go to the protocol tab on the top and write transcription see what you get.




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