Phage removal of E.coli
Posted 08 July 2009 - 05:00 AM
Does anyone know of a protocol to do this? What amounts, what conditions etc?
Thank you very much for your help.
Posted 08 July 2009 - 05:39 AM
Posted 08 July 2009 - 10:10 AM
Posted 08 July 2009 - 10:33 AM
then heaven will be yours, before you meet your end
Posted 08 July 2009 - 04:45 PM
A better way might be to use a modified E. coli that is an auxotroph. That way, the bugs can be killed by removing the essential compound.
Posted 09 July 2009 - 07:12 AM
I like this idea, I'll see if I can track it down.
My PI is rather attached to the Phage idea so perhaps I will try both, either way, just for future reference, how does one go about getting their hands on some Phage? I would think that it would be sold from some biotech company but I have yet to track some down.
Am I missing an obvious resource?
Posted 09 July 2009 - 07:20 AM
We use E. coli to conjugate plasmids to our bacterium (another enteric, but not E. coli), and to select conjugates, we plate on 10 ug/ml colistin to remove E. coli. Works very well, and it's quite easy.
The only caveat would be whether or not your species is colistin resistant.
Edited by fishdoc, 09 July 2009 - 07:22 AM.
Posted 09 July 2009 - 08:21 AM
Posted 09 July 2009 - 05:20 PM