Posted 08 July 2009 - 02:25 AM
I've been trying very unsuccessfully to transform a lux plasmid into a staph strain.
I'm trying to go the transduction route now instead.
What I am wondering is how to go about it? Does anyone have a staph specific protocol or failing that a gram positive protocol or maybe an informative link??
Posted 08 July 2009 - 05:24 AM
Posted 08 July 2009 - 10:13 PM
The plasmid was specifically made for staph strains. my prob is that I'm trying to transform clinical isolates and it's just not working out. I've been trying for several months with several different protocols and getting no where ????
Posted 09 July 2009 - 04:26 PM