Posted 07 July 2009 - 10:35 PM
Posted 15 July 2009 - 02:53 PM
A common buffer for cell lysis prior to Luciferase is CCLR buffer, which is 100 mM potassium phosphate, pH 7.8, 1 mM EDTA, 7 mM 2-mercaptoethanol, 1% (v/v) Triton X-100, and 10% (v/v) glycerol.
I don't know the recipe for stop solution, sorry.
Reliable laboratory instruments for the life sciences, designed to enable you to work more effectively and increase productivity. Check us out!
If my answers help you, please take a moment to check out our site and see if any of our products would help you as well! Thanks!
Posted 30 September 2009 - 05:38 AM
"This is SPARTA!"
"I´m the goddamn batman"
Posted 02 December 2009 - 03:54 PM
Hampf M, Gossen M.
Anal Biochem. 2006 Sep 1;356(1):94-9.
A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays.
---my blog on reporter genes----