Hi, i like to know if anyone has a suitable recipe for lysis buffer that could be used to lyse cells for luciferase assay not those from commercial kits. Also anyone has recipe for the stop solution for luciferase assay. Thanks in advance
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#2
Carlton H
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Posted 15 July 2009 - 02:53 PM
Candice,
A common buffer for cell lysis prior to Luciferase is CCLR buffer, which is 100 mM potassium phosphate, pH 7.8, 1 mM EDTA, 7 mM 2-mercaptoethanol, 1% (v/v) Triton X-100, and 10% (v/v) glycerol.
I don't know the recipe for stop solution, sorry.
-Carlton
A common buffer for cell lysis prior to Luciferase is CCLR buffer, which is 100 mM potassium phosphate, pH 7.8, 1 mM EDTA, 7 mM 2-mercaptoethanol, 1% (v/v) Triton X-100, and 10% (v/v) glycerol.
I don't know the recipe for stop solution, sorry.
-Carlton
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#3
laurequillo
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Posted 30 September 2009 - 05:38 AM
you can use normal np-40 lysis buffer
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#4
96well
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Posted 02 December 2009 - 03:54 PM
have a glance at
Hampf M, Gossen M.
Anal Biochem. 2006 Sep 1;356(1):94-9.
A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays.
96well
---my blog on reporter genes----
Hampf M, Gossen M.
Anal Biochem. 2006 Sep 1;356(1):94-9.
A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays.
96well
---my blog on reporter genes----
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