plasmid rescue (plasmids with incompatible ORI)
Posted 07 July 2009 - 08:18 PM
I'm trying to ligate two plasmids (one with kanamycin and other with ampicillin selection markers) containing similar (pMB1 and ColE1) ORI's. I think as they fall in the same plasmid incompatibility group, I don't get any transformants on LB Kan/Amp selection plate. I'm using DH5 alpha chemically competent cells for transformation. Can someone please suggest if there is any bacterial strain where in a plasmid with two similar ORI's can exist? Or is there any other way to get this work done.
Thanks a lot
Posted 07 July 2009 - 10:08 PM
Posted 08 July 2009 - 07:31 AM