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Better ideas than Re-Chip


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7 replies to this topic

#1 yg_eagle

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Posted 07 July 2009 - 08:15 AM

Hi, all experts, I am doing re-Chip now. My purpose is to find most of the TFs (transcription factors, at least those binded on DNA) in my interested chromatin region. Now I only think out re-Chip followed by co-IP to meet it. But you know, we can only check one TF in one re-Chip and somebody told me re-Chip is much harder than Chip. So, does anyone have better ideas than re-chip? Or can you give me some suggestions if you are expert in re-Chip?
Thanks a lot for your great help in advance!
Ge

#2 cellcounter

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Posted 07 July 2009 - 10:06 AM

Hi, all experts, I am doing re-Chip now. My purpose is to find most of the TFs (transcription factors, at least those binded on DNA) in my interested chromatin region. Now I only think out re-Chip followed by co-IP to meet it. But you know, we can only check one TF in one re-Chip and somebody told me re-Chip is much harder than Chip. So, does anyone have better ideas than re-chip? Or can you give me some suggestions if you are expert in re-Chip?
Thanks a lot for your great help in advance!
Ge

Re-ChIP is two ChIP in one, so obviously it is more difficult.

Not necessarily a better idea, but other idea would be to add one more crosslinker -such as DTBP.

#3 yg_eagle

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Posted 07 July 2009 - 10:55 AM

Hi, cellcounter, thank you so much for your reply. But how and in which step to add one more crosslinker? And what is the meaning of DTBP?
Ge

#4 KPDE

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Posted 07 July 2009 - 02:13 PM

Hi, cellcounter, thank you so much for your reply. But how and in which step to add one more crosslinker? And what is the meaning of DTBP?
Ge


Dimethyl 3,3-dithiobispropionimidate

#5 yg_eagle

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Posted 08 July 2009 - 02:57 PM

Hi, cellcounter, thank you so much for your reply. But how and in which step to add one more crosslinker? And what is the meaning of DTBP?
Ge


Dimethyl 3,3-dithiobispropionimidate


Hi, KPDE, thank you very much for your explanation. But could you tell me how to use DTBP? Add it with formaldehyde? If your know where I can get a detailed re-Chip protocol, that is best. I just wonder if there are some knacks in sides to get a better re-chip results.
Thanks again!
Ge

#6 BioBus

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Posted 09 July 2009 - 06:24 AM

Hi, cellcounter, thank you so much for your reply. But how and in which step to add one more crosslinker? And what is the meaning of DTBP?
Ge


Dimethyl 3,3-dithiobispropionimidate


Hi, KPDE, thank you very much for your explanation. But could you tell me how to use DTBP? Add it with formaldehyde? If your know where I can get a detailed re-Chip protocol, that is best. I just wonder if there are some knacks in sides to get a better re-chip results.
Thanks again!
Ge


google /NCBI

Wade P , MTA3 ,DTBP

#7 cellcounter

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Posted 09 July 2009 - 06:40 AM

Hi, cellcounter, thank you so much for your reply. But how and in which step to add one more crosslinker? And what is the meaning of DTBP?
Ge


Dimethyl 3,3-dithiobispropionimidate


Hi, KPDE, thank you very much for your explanation. But could you tell me how to use DTBP? Add it with formaldehyde? If your know where I can get a detailed re-Chip protocol, that is best. I just wonder if there are some knacks in sides to get a better re-chip results.
Thanks again!
Ge

A few protocols.

#8 yg_eagle

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Posted 09 July 2009 - 10:04 PM

Hi, Biobus and Cellcounter, I got it. Thank you guys very much for kind help!
Ge




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