7 replies to this topic

[yg_eagle]

Hi, all experts, I am doing re-Chip now. My purpose is to find most of the TFs (transcription factors, at least those binded on DNA) in my interested chromatin region. Now I only think out re-Chip followed by co-IP to meet it. But you know, we can only check one TF in one re-Chip and somebody told me re-Chip is much harder than Chip. So, does anyone have better ideas than re-chip? Or can you give me some suggestions if you are expert in re-Chip?
Thanks a lot for your great help in advance!
Ge

[cellcounter]

yg_eagle, on Jul 7 2009, 09:15 AM, said:

Hi, all experts, I am doing re-Chip now. My purpose is to find most of the TFs (transcription factors, at least those binded on DNA) in my interested chromatin region. Now I only think out re-Chip followed by co-IP to meet it. But you know, we can only check one TF in one re-Chip and somebody told me re-Chip is much harder than Chip. So, does anyone have better ideas than re-chip? Or can you give me some suggestions if you are expert in re-Chip?
Thanks a lot for your great help in advance!
Ge

Re-ChIP is two ChIP in one, so obviously it is more difficult.

Not necessarily a better idea, but other idea would be to add one more crosslinker -such as DTBP.

[yg_eagle]

Hi, cellcounter, thank you so much for your reply. But how and in which step to add one more crosslinker?  And what is the meaning of DTBP?
Ge

[KPDE]

yg_eagle, on Jul 7 2009, 11:55 AM, said:

Hi, cellcounter, thank you so much for your reply. But how and in which step to add one more crosslinker?  And what is the meaning of DTBP?
Ge

Dimethyl 3,3-dithiobispropionimidate

[yg_eagle]

KPDE, on Jul 7 2009, 03:13 PM, said:

yg_eagle, on Jul 7 2009, 11:55 AM, said:

Hi, cellcounter, thank you so much for your reply. But how and in which step to add one more crosslinker? And what is the meaning of DTBP?
Ge

Dimethyl 3,3-dithiobispropionimidate

Hi, KPDE, thank you very much for your explanation. But could you tell me how to use DTBP? Add it with formaldehyde? If your know where I can get a detailed re-Chip protocol, that is best. I just wonder if there are some knacks in sides to get a better re-chip results.
Thanks  again!
Ge

[BioBus]

yg_eagle, on Jul 8 2009, 03:57 PM, said:

KPDE, on Jul 7 2009, 03:13 PM, said:

yg_eagle, on Jul 7 2009, 11:55 AM, said:

Hi, cellcounter, thank you so much for your reply. But how and in which step to add one more crosslinker? And what is the meaning of DTBP?
Ge

Dimethyl 3,3-dithiobispropionimidate

Hi, KPDE, thank you very much for your explanation. But could you tell me how to use DTBP? Add it with formaldehyde? If your know where I can get a detailed re-Chip protocol, that is best. I just wonder if there are some knacks in sides to get a better re-chip results.
Thanks  again!
Ge

google /NCBI

Wade P , MTA3 ,DTBP

[cellcounter]

yg_eagle, on Jul 8 2009, 03:57 PM, said:

KPDE, on Jul 7 2009, 03:13 PM, said:

yg_eagle, on Jul 7 2009, 11:55 AM, said:

Hi, cellcounter, thank you so much for your reply. But how and in which step to add one more crosslinker? And what is the meaning of DTBP?
Ge

Dimethyl 3,3-dithiobispropionimidate

Hi, KPDE, thank you very much for your explanation. But could you tell me how to use DTBP? Add it with formaldehyde? If your know where I can get a detailed re-Chip protocol, that is best. I just wonder if there are some knacks in sides to get a better re-chip results.
Thanks  again!
Ge

A few protocols.

[yg_eagle]

Hi, Biobus and Cellcounter, I got it. Thank you guys very much for kind help!
Ge