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Number of cells in 6 wells-plate well

Started by Neeltje, Jul 07 2009 07:34 AM

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#1 Neeltje

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Posted 07 July 2009 - 07:34 AM

I am doing some growth curves of virus in MDCK cells. Does anyone have an idea how many cells there generally are in a well of a 6-wells plate, or perhaps how many MDCK cells there will be?
Thanks,
Neeltje

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#2 cellcounter

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Posted 07 July 2009 - 08:55 AM

Neeltje, on Jul 7 2009, 07:34 AM, said:

I am doing some growth curves of virus in MDCK cells. Does anyone have an idea how many cells there generally are in a well of a 6-wells plate, or perhaps how many MDCK cells there will be?
Thanks,
Neeltje

The best way to find out is by counting cells in a well.

There is really no general thing, because it depends upon confluence, and over-confluence of cells, especially in MDCK cells. They can really overgrow even after reaching confluence.