Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Na+/K+ ATP ase Assay


  • Please log in to reply
No replies to this topic

#1 FlicFlak

FlicFlak

    member

  • Active Members
  • Pip
  • 6 posts
1
Neutral

Posted 07 July 2009 - 06:02 AM

Hi everyone, first post!! Long time reader though.
Was just wondering if anyone has done the above assay?
The protocol im using states:
To determine the amount of phosphate produced, add 1.5 ml of a freshly prepared solution containing 9.6% (w/v) FeSO4.7H2O and 1.5% (w/v) ammonium heptamolybdate in 0.66 M H2SO4 to both standards and samples.
When i make it up in the order i get a blue/black solution which is unusuable but if i reverse it (add the molybdate then add the iron sulfate) i get a copper brown solution which i then add to my samples (Brush border membrane vesicles and Homogenate) and this detects the inorganic phosphate which give a blue colour.
My question is, why does it make a difference which substance is added first and has anyone else come accross this?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.