I am looking for a vector within which I can put both my gene of interest and EGFP but under different promoters. My PI is pretty insistent that they're not under the same promoter eg gene of interest driven by CMV and EGFP driven by ubiquitin promoter. Does such a vector exist? I read something about bidirectional vectors where one promoter can drive the expression of 2 genes in different directions but I cant seem to find them - are they commercially available?
The vector will ultimately be used for transient transfection of cancer cell lines. Any help would be greatly appreciated.
Edited by moorele, 07 July 2009 - 04:33 AM.