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does 1% SDS prevents proteases?


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8 replies to this topic

#1 yobou

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Posted 06 July 2009 - 09:56 AM

Dear All
a colleague claims that 1% SDS in lysis buffer is sufficient to inhibit proteases and there is no need to use protease inhibitors. is this correct?

#2 hobglobin

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Posted 06 July 2009 - 11:28 AM

Dear All
a colleague claims that 1% SDS in lysis buffer is sufficient to inhibit proteases and there is no need to use protease inhibitors. is this correct?

At least Proteinase K is not inhibited...

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

That is....if she posts at all.


#3 bob1

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Posted 06 July 2009 - 04:25 PM

It is false - SDS is a detergent, it primarily dissolves lipids rather than proteins.

Edited by bob1, 06 July 2009 - 04:26 PM.


#4 HomeBrew

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Posted 06 July 2009 - 07:23 PM

Proteinase K's activity is actually enhanced by SDS: "Activity can be stimulated by addition of denaturing agents (SDS and urea)" (see here, under "Activators").

#5 yobou

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Posted 07 July 2009 - 07:28 AM

Proteinase K's activity is actually enhanced by SDS: "Activity can be stimulated by addition of denaturing agents (SDS and urea)" (see here, under "Activators").

I mean proteases in human cancer cells (serine, cysteine and aaspartate , ...etc proteaes) . I guess proteinaseK is not among them.

#6 HomeBrew

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Posted 07 July 2009 - 02:48 PM

Proteinase K is a serine protease from a eukaryotic organism...

Regardless, in answer to your question -- I don't think 1% SDS is effective enough to rely on it alone for protease inhibition.

#7 GeorgeWolff

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Posted 08 July 2009 - 01:36 AM

Ask your colleague for the data.

#8 yobou

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Posted 08 July 2009 - 12:05 PM

Ask your colleague for the data.

actually before I open this topic for discussion I did so, and he showed me a blot for a protein that is clealry upregulated by certain drug treatment as a positive control. That is why I was asking whether this method is valid or not.

Edited by yobou, 08 July 2009 - 12:06 PM.


#9 Tfal

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Posted 17 July 2009 - 04:22 AM

is there any reason you wish to avoid habitual protease inhibitors?




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