hi
how effective is EDTA as denaturing agent for proteins???
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#2
mdfenko
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Posted 06 July 2009 - 08:22 AM
if you have a metallo-protein then it may denature the protein by removing the metal.
otherwise, i have never heard of its use in the denaturing of protein.
otherwise, i have never heard of its use in the denaturing of protein.
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#3
HomeBrew
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Posted 06 July 2009 - 01:40 PM
It is frequently used in protein storage buffers...
Other than some specialized proteins that require coordination with various metal ions to retain their state (as mdfenko points out), I wouldn't consider EDTA a denaturing agent.
Other than some specialized proteins that require coordination with various metal ions to retain their state (as mdfenko points out), I wouldn't consider EDTA a denaturing agent.
#4
rick112
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Posted 10 July 2009 - 01:00 AM
thanks HomeBrew and mdfenko for the reply...
i also had similar opinion its just that one of my colleague had such a strong view on this...that i felt i must conform it...
ok..lets just trun the question little around
what makes 'Urea' and 'GuHCL (Guanidine Hydrochloride)' such a good denaturing agents for proteins??
i also had similar opinion its just that one of my colleague had such a strong view on this...that i felt i must conform it...
ok..lets just trun the question little around
what makes 'Urea' and 'GuHCL (Guanidine Hydrochloride)' such a good denaturing agents for proteins??
#5
mdfenko
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Posted 10 July 2009 - 06:35 AM
this comes from wikipedia:
urea breaks non-covalent bonds.
guanidinium chloride is a powerful protein denaturant. wiki references a book from 1978:
Lapange, Savo (1978). Physicochemical aspects of protein denaturation. New York: Wiley. ISBN 0471034096.
you can also get information on protein denaturation in the handbook of chemistry and physics.
urea breaks non-covalent bonds.
guanidinium chloride is a powerful protein denaturant. wiki references a book from 1978:
Lapange, Savo (1978). Physicochemical aspects of protein denaturation. New York: Wiley. ISBN 0471034096.
you can also get information on protein denaturation in the handbook of chemistry and physics.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#6
hobglobin
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Posted 10 July 2009 - 11:22 AM
It inactivates enzymes that need bivalent metal ions as coenzymes. But this is not a denaturation....
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
