RNA quantification in microplate reader
Posted 02 July 2009 - 05:28 PM
i.e. read absorbance in a microplate reader at 260 and 280nm instead of a cuvette
Is this possible? How would I calculate the concentration?
Posted 02 July 2009 - 05:33 PM
Posted 03 December 2009 - 11:28 AM
Posted 03 December 2009 - 08:11 PM
Only thing that has to be taken care is to set the path length corerction as by changing the volume, tht path length wll change in a microplate reader. Most readers have this facility of path legth correction