Our lab uses sodium borate gels for electrophoresis. I am using Qiagen GelPilot loading dye and the handbook/protocol includes the migration rates of the dyes in TAE and TBE buffer, but not in sodium borate buffer. Are the migration rates similar to either TAE or TBE, or will they be different?
0
1 reply to this topic
#2
swanny
-
- Active Members
-
- 367 posts
Veteran
7
Neutral
Neutral
Posted 02 July 2009 - 03:43 PM
Neotoma, on Jul 3 2009, 03:39 AM, said:
Our lab uses sodium borate gels for electrophoresis. I am using Qiagen GelPilot loading dye and the handbook/protocol includes the migration rates of the dyes in TAE and TBE buffer, but not in sodium borate buffer. Are the migration rates similar to either TAE or TBE, or will they be different?
There shouldn't be too much difference. Just run it about halfway down and have a look. Continue as appropriate.
Edited by swanny, 02 July 2009 - 03:44 PM.
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.
